The tornado data in Jiangsu Province and high resolution reanalysis dataset were used to investigate the characteristics of tornado occurrence and the influence of ENSO events on the tornado in Jiangsu Province. Results show that:(1) the occurrence of tornado has significant annual and inter-decadal variation as well as spatial differences. Tornado occurred more in 1990s, moreover, the south of Yancheng and the coastal region of Nantong are the main regions of high occurrence of tornado. (2) The ENSO events have significant influence on the occurrence of tornado in Jiangsu Province. During negative ENSO phases, the number of tornado exceeds positive phases and in non-ENSO period, the occurrences of tornado in Jiangsu is between positive and negative ENSO phases. (3) The number of southwesterly low-level jet are higher in negative ENSO events leading to stronger water supply and instable energy with the more advantageous dynamic characteristics, which has a positive impact on the occurrence of tornado.