Based on the known cloud vertical structure judgment method, combined with the relative humidity threshold method and the temperature dew point method, a more scientific and effective method was obtained. Moreover, the radiosonde data in Nanjing was used to collect the cloud vertical structure parameter of Nanjing, in order to statistically analyze the cloud vertical structure features. It was found that this new method is feasible; cloud occurs more frequently in summer and autumn in Nanjing than in spring and winter, especially for the multilayer cloud; and the cloud thickness is thicker in summer than in winter and decreases with the cloud height.